| 1. | Construction of recombinant lactococcus lactis expressing porcine transmissible gastroenteritis spike glycoprotein and analysis of immunogenicity
基因的重组乳酸乳球菌的构建及免疫原性分析 |
| 2. | Sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored little ability of nisin production
以lactococcuslactisnz9800为受体菌，研究了表达载体的表达情况。 |
| 3. | Using a mouse model , the uk team has also found that a modified bacterium lactococcus lactis can be used to vaccinate against harmful infections
利用小鼠模型，该英国研究小组已经发现了一种基因修饰细菌（乳酸乳球菌）可用于对有害传染病的免疫接种。 |
| 4. | 4 . the recombinant plasmid pmg36e / nisa was introduced into lactococcus lactis nz9800 . sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored no ability of nisin production
抽提ecolijm109中的重组质粒，转化l . lactisnz9800 ，在含红霉素的gm17平板上筛选转化子。 |
| 5. | This has resulted in the availability of genetically modified l . lactis with prospects for application in food industry . in this studay , genomic dna extracted from lactococcus lactis nizo r5 was used directly as the template for pcr in this paper
乳酸乳球菌是一类长期应用于食品发酵业的有益微生物，以乳酸菌为受体的表达系统具有安全、表达产物便于分离，使用方便等优点。 |
| 6. | A dna fragment was amplified from lactococcus lactis nizo r5 genome by means of pcr and cloned into pmg36e plasmid . the recombinant plasmid was transformd into e . colijm 109 and identified by restriction endonucleases analyzing . the recombinant plasmid was introduced into lactococcus lactis nz9800
本实验提取乳酸乳球菌nizor5的基因组dna为模板，用pcr方法得到乳链菌肽前体基因( nisa ) ，克隆到乳酸乳球菌表达载体pmg36e ，构建了表达载体pmg36e nisa 。 |
| 7. | A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome . the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 " . conditions used for the pcr consisted of 30 cycles of 94 ? for0 . 5min , 45 ? for imin and 72 ? for 1 . 5min , plus one additional cycle of 72 for 10min
根据已发表的乳链菌肽前体基因的dna序列，设计两条引物并引入酶切位点。以lactococcuslactisr5基因组dna为模板， pcr反应条件： 94变性30s ， 45退火60s ， 72延伸90s ，反应进行30个循环。成功扩出一条约460bp的dna片段。 |
